Murashige & Skoog (Modification No. 5) Macroelements with NH4NO3 replaced by NaNO3, is supplied by HiMedia. It is available in the form of a white to off-white, homogenous, free flowing powder with a pH in the range of 4.4 to 5.4. Since this product is extremely hygroscopic, it is advisable to store it at 2-8C, in air tight containers. The composition (mg/ml) is as follows:

Potassium nitrate (1900.00)

Calcium chloride.2H2O (440.00)

Magnesium sulphate (180.69)

Potassium phosphate monobasic (170.00)

Sodium nitrate (1751.00)

Murashige and Skoog Medium (MS) was originally formulated by Murashige and Skoog in 1962 to optimize tobacco callus bioassay system for facilitating the study of cytokinins. It is commonly used for micro propagation, organ culture, callus culture and suspension culture. Murashige & Skoog (Modification No. 5) macroelements has been specially formulated for plant cell, tissue and organ cultures. Potassium nitrate and sodium nitrate serves as the sources of nitrate.