Nitrate Reagent-I (Sulphanilic Acid-0.8%) is supplied by Micromaster. It is available in the form of a very light amber coloured solution with characteristic odour. It has a pH in the range of 1.50-1.70. This product should be stored between 10-30C in a tightly closed container.

Quantity provided: 100 ml/bottle

It is used along with Alpha- Naphthylamine solution for determination of nitrate reduction by bacterial strains. The a-Naphthylamine solution and Sulphanilic acid is used to determine nitrate reduction by bacterial strains. The reduction of nitrates (NO3) leads to the formation of nitrites (NO2) and may progress to the liberation of nitrogen gas. The nitrate reductase producing organisms reduce nitrate to nitrite which reacts with sulphanilic acid to form a diazonium salt. This salt reacts with a-naphthylamine to form a red coloured, water soluble azo dye which results in the visualization of pink-red colour. A distinct red colour formation within 1-2 minutes indicates reduction of nitrate to nitrite.