Pertussis toxin was first isolated from B. pertussis and is used to study G protein-coupled receptor signaling in cells and experimental autoimmune encephalomyelitis (EAE) in animals. Pertussis toxin catalyzes the transfer of the ADP-ribose moiety of NAD to the subunits of heterotrimeric Gi/o proteins, resulting in the receptors being uncoupled from Gi/o proteins.1,2 Pertussis toxin is also used as an adjuvant, given with specific antigens, to immunize animals and induce EAE, an animal model of multiple sclerosis.3,4Technical InformationPurity95% (estimated by SDS-PAGE)