Specific antibodies are used commonly for the identification and characterization of proteins during techniques such as ELISA, immune-electrophoresis, western blotting etc. These antibodies can be monoclonal or polyclonal, based on the requirement of the assay. Antibodies against a particular protein can be raised in various species of animals (hosts). The host animals are immunized with the protein and the antibodies generated in the host serum against the protein are collected and purified for use. The antibodies can also be conjugated to several labels, which can be used to detect the antibodies bound to specific target antigens for quantitative and qualitative analysis. The Rabbit anti mouse IgG HRP is a conjugated secondary antibody from Genei. This antibody is generated using rabbit as a host. IgG is procured from mouse serum and is injected into rabbit, as an immunogen. The antibody generated in the rabbit is isolated and purified via affinity chromatography. To enable its use as a secondary antibody, this product is conjugated to HRP (horse-radish peroxidase). This enzyme uses hydrogen peroxidase to catalyse the oxidation of several substances, such as TMB. To quantify the amount of bound HRP conjugated antibody, the substrate must be added along with hydrogen peroxide. The resulting coloured product can be quantified using spectrometric methods.
Product name: Rabbit anti-mouse IgG HRP conjugated
Quantity: 1 mL
Catalogue number: 1140580011730