Spoligotyping is a PCR- based Reverse Line Hybridization method to simultaneously detect and type Mycobacterium tuberculosis complex bacteria in clinical samples, exploiting polymorphism in the "Direct Repeat" (DR) region, which is uniquely present in Mycobacterium tuberculosis complex bacteria which includes Mycobacterium tuberculosis, M. africanum, M. bovis, M. canettii, M. microti, M. caprae, and M. pinnipedii. The presence or absence of the strains can be detected by hybridization of PCR amplified sample to a set of immobilized oligonucleotides on the membranes.
Spoligotyping Kit (IM9701) consists of four vials (2 controls & 2 primers), One membrane and a manual. Membrane is bound with spacer Oligonucleotides. The membrane is shipped in 20 mM EDTA.
Two vials containing the positive controls:
a) positive control 1 (M. tuberculosis strain H37Rv)
b) positive control 2 (M. bovis BCG P3)
Two vials containing the primers for PCR amplification:
a) primer Dra (biotinylated)
b) primer Drb