Tudor domains (or tudor-like regions) are small protein structural motifs of ~50 amino acids related to the Royal Family of methyl rEAders, which also includes chromo, malignant brain tumor (MBT), PWWP, and Agenet-like domains.1,2 Tudor domains occur either alone, in tandem, or with other domains and are found in many proteins that are involved in RNA metabolism, germ cell development, transposon silencing, DNA damage response, histone modification, and chromatin remodeling.3 The tudor domains recognize symmetric methylated arginine or methylated lysine residues.4,5,6,7 Ubiquitin-like with PHD and ring finger domains 1 (UHRF1) is a multidomain-containing nuclEAr protein known to bind chromatin and participate in the maintenance of DNA methylation.8,9 The SET and RING associated domain of UHRF1, also called the YDG motif, binds methyl cytosines, while trimethylated histone H3 lysine 9 (H3K9me3) and unmethylated histone H3 Arginine 2 (H3R2me0) are recognized by the tandem tudor-like domains and the PHD domain, respectively.10,11,12,13,14,15 Some evidence suggests the tandem tudor-like region and adjacent PHD domain may operate together to recognize H3K9me3.16 The combinatorial recognition of the histone tail region and hemi-methylated DNA functions to regulate gene silencing by directly interacting with DNA (cytosine-5)-methyltransferase 1.17,8,18 UHRF1 also posseses E3 ubiquitin ligase activity toward histone H3 and the tumor suppressor promyelocytic leukemia protein.13,19 Binding of UHRF1 tandem tudor-like domains to H3K9me3 is involved in heterochromatin formation and maintenance.13 This protein product contains the tudor-like region of UHRF1.Technical InformationSynonymsE3 Ubiquitin-protein Ligase UHRF1Inverted CCAAT Box Binding Protein of 90 kDaNuclEAr Protein 95RING Finger Protein 106Transcription Factor ICBP90Ubiquitin-like PHD and RING Finger Domain-containing Protein 1Purity95% estimated by SDS-PAGESourceRecombinant protein expressed in E. coliAmino Acids126-285 (partial protein)MW31.9 kDaFormulation50 mM Tris, pH 8.0, containing 150 mM sodium chloride, 2.2 mM potassium chloride, 10 mM -mercaptoethanol, and 20% glycerolLicenseSUMOpro tag was used under non-exclusive license from LifeSensors, Inc.UniProt Accession Q96T88