Agar Medium F-E-500g
- Brand: Micromaster
- Catalog No.: DM1327E-500g
- Quantity/Unit: 500g/Pack
- Usually Shipped in: 1-2 Weeks
Min Orderable Qty : 1 Pack
For lab/research use only, unless otherwise specified
High grade Agar Medium F is supplied by Micromaster. It is available in the form of a light yellow to pink homogenous free flowing powder which has a pH in the range of 7.2 to 7.6. It is advisable to store this product between 10-30C in a tightly closed container. Once opened, Agar Medium F should be properly stored in a dry place, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Store this product in dry ventilated area, away from extreme temperatures and sources of ignition.
Quantity provided: 500g /pack
Agar Medium F is a selective medium, recommended for detection and enumeration of Enterobacteria in accordance with European Pharmacopoeia. It is also referred to as Crystal Violet, Neutral Red, Bile Agar with Glucose. Mossel et al added glucose to the medium after observing an improved detection of coliforms. Incubation can be carried out at different temperatures and incubation time depending upon the group of Enterobacteriaceae to be recovered. Gelatin and yeast extract provide nitrogenous and carbonaceous compounds, long chain amino acids, vitamins and other nutrients essential for bacterial metabolism. The presence of the inhibitors along with bile salts and crystal violet makes this media selective. Crystal violet inhibits gram-positive organisms especially Staphylococci. Neutral red indicator helps to detect lactose monohydrate and glucose monohydrate fermentation. Sodium chloride aids in maintaining the osmotic equilibrium in the medium. The red colour is due to absorption of neutral red and a subsequent colour change of the dye when the pH of medium falls below 6.8.
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