Chloroform, 99% is supplied by Avra Synthesis Pvt. Ltd. It contains a colourless, clear, dense, non-flammable liquid, which has a pleasant odour.

Molecular Formula: CHCl3

Molecular Weight: 119.4

CAS Number: 67-66-3

Density: 1.484 g/ml (20 C)

Boiling Point: 61 - 62 C

Melting Point: -63.5 C

Synonym: Trichloromethane, Methylidine trichloride.It is used mostly for extraction of nucelic acids.Chloroform is widely used in molecular biology and biochemistry experiments.It is used as a solvent, because of its raltive unreactivity and miscibility with most organic liquids and organic synthesis. It is used in industrially in the preparation of chlorodifluoromethane (HCFC-22), a propellant and refrigerant. It is also used in purification of DNA and penicillin.It is known to be used in variety of procedures like cDNA Storage, PCR reaction and procedure, oligonucleotide purification., etc.Chloroform has been used in many industrial protocols like high-performance thin-layer chromatography protocol for sphingomyelin analysis.It is also reported being used in analysis and extraction in Mass- Spectrometry.