Dey-Engley Neutralizing Broth is supplied by Micromaster. It is available in the form of a light yellow to bluish grey homogeneous free flowing powder with a pH in the range of 7.4 to 7.8. It is advisable to store this product below 30C in tightly closed container. Always use freshly prepared medium.

Quantity provided: 100 g/pack

Dey-Engley Neutralizing Broth is formulated as per the procedure stated by Engley and Dey. It is specially suited for environmental sampling where neutralization of the chemical is important to determine its bactericidal activity. A strongly bacteriostatic substance inhibits the growth and reproduction of bacteria without killing them. These bacteria hold the ability to cause infection under favourable conditions. The Dey-Engley Neutralizing Broth neutralizes a broad spectrum of antiseptics and disinfectants including quaternary ammonium compounds, phenolics, iodine and chlorine preparations, mercurials, formaldehyde and glutaraldehyde. Tryptone provides nitrogen and carbon source, long chain amino acids, vitamins and other essential nutrients. Dextrose is an energy source. Yeast extract is also a rich source of vitamin B-complex. The present formulation incorporates neutralizing substances for almost all the active products used as antiseptics and disinfectants. Sodium bisulfite neutralizes aldehydes; sodium thioglycollate neutralizes mercurials; sodium thiosulfate neutralizes iodine and chlorine; lecithin neutralizes quaternary ammonium compounds; and polysorbate 80, a non-ionic surface-active agent, neutralizes substituted phenolics. Bromocresol purple is an indicator for dextrose utilization. The high concentration of lecithin in the broth medium, makes it difficult to use turbidity to detect growth. Hence, bromocresol purple and dextrose are added to the medium. Those organisms that ferment dextrose will turn the medium from purple to yellow.