Dichloran Medium Base W/ Rose Bengal
- Brand: Micromaster
- Catalog No.: DM1068-500g
- Quantity/Unit: 500g/Pack
- Usually Shipped in: 1-2 Weeks
Min Orderable Qty : 1 Pack
For lab/research use only, unless otherwise specified
High-grade Dichloran Medium Base with Rose Bengal is supplied by Micromaster. It is available in the form of a light yellow to pink homogenous free-flowing powder which has a pH in the range of 5.4 to 5.8. For maximum efficiency, it must be stored below 30C in a tightly closed container.
The quantity provided: 500g /pack
Dichloran Medium Base with Rose Bengal is formulated as per the description is given by King et al, which is nothing but a modification of Rose Bengal Chloramphenicol Agar. Dichloran Medium Base with Rose Bengal is used along with Rose Bengal Chloramphenicol Agar in situations where enumeration of yeasts is required in the presence of molds. The peptic digest of animal tissue provides nitrogen, vitamins, and minerals. Dextrose is a carbohydrate source. Phosphate buffers the medium. Magnesium sulfate provides divalent cations and sulfate. Dichloran is an antifungal agent, added to the medium to reduce colony diameters of spreading fungi. Rose bengal exhibits an improved inhibitory activity at pH 5.6 and therefore the final pH of the medium is reduced to 5.6 for the inhibition of spreading fungi. The presence of rose bengal in the medium suppresses the growth of bacteria and restricts the size and height of colonies of the more rapidly growing molds. The concentration of rose bengal is reduced for optimal performance with dichloran. Additionally, rose bengal is taken up by yeast and mold colonies, which allows these colonies to be easily recognized and enumerated.
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