DMEM w/ L-Glutamine and Sodium pyruvate w/o Glucose and Sodium bicarbonate

DMEM w/ L-Glutamine and Sodium pyruvate w/o Glucose and Sodium bicarbonate

₹ 4,700.00 *

Brand: HIMEDIA

Catalog No.: AT186-20L

Quantity/Unit: 20lt/EA

Usually Shipped in: 2-3 Weeks

Qty:
Min Orderable Qty : 1 Pack

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Product Description

Dulbecco's Modified Eagle Medium (DMEM) With L-Glutamine and Sodium pyruvate Without Glucose and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 5.9 -6.5 whereas the pH with Sodium Bicarbonate is between 7.5-8.1. The osmolality without Sodium Bicarbonate is in the range of 230.00 -270.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 310.00 -350.00 Osm/Kg H20. It is advisable to store this product between 2-8⁰C. Media composition (in mg/L) is as follows:

• Calcium chloride dihydrate (265.00)

• Ferric nitrate nonahydrate (0.10)

• Magnesium sulphate anhydrous (97.72)

• Potassium chloride (400.00)

• Sodium chloride (6400.00)

• Sodium dihydrogen phosphate anhydrous (109.00)

• Amino acids

• Choline chloride (4.00)

• D-Ca-Pantothenate (4.00)

• Folic acid (4.00)

• Nicotinamide (4.00)

• Pyridoxal hydrochloride (4.00)

• Riboflavin (0.400)

• Thiamine hydrochloride (4.00) 

• i-Inositol (7.20)

• Phenol red sodium salt (15.90)

• Sodium pyruvate (110.00)

Dulbecco's Modified Eagle Medium is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four-fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mg glucose per litre and was originally used to culture embryonic mouse cells. DMEM high glucose is a further modification of original DMEM and contains 4.5gms/L of glucose. The additional glucose has proved to be useful in cultivating various other cell lines including primary cultures of mouse and chicken cells as well as various normal and transformed cell lines. This medium contains L-glutamine and Sodium pyruvate but does not contain glucose and sodium bicarbonate. 

 

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