High grade Fluid Thioglycollate Medium (Thioglycollate Medium, Fluid) is supplied by Micromaster. It is available in the form of a cream to yellow homogenous free flowing powder with a pH in the range of 6.9 to 7.3. This product should be stored between 10-30C in a tightly closed container. Once opened, it should be properly stored, after tightly capping the bottle. Due to the hygroscopic nature of the product, there could be chances of lump formation which can be avoided by proper storage. Store in dry ventilated area protected from extremes of temperature and sources of ignition.

• Quantity provided: 500 g/pack

Fluid Thioglycollate medium is used for sterility testing of biologicals and for cultivation of anaerobes, aerobes and microaerophiles in compliance with EP and BP. Brewer formulated Fluid Thioglycollate Medium for rapid cultivation of aerobes as well as anaerobes including microaerophiles by adding a reducing agent and small amount of agar. This media is recommended for sterility testing of antibiotics, biologicals and foods and for determining the phenol coefficient and sporicidal effect of disinfectants. However, it is intended for the examination of clear liquid or water-soluble materials. Fluid Thioglycollate Medium is also routinely used to check the sterility of stored blood in blood banks. Dextrose, tryptone, yeast extract, L-cystine provide the growth factors necessary for bacterial multiplication. L-cystine and sodium thioglycollate allows Clostridium to grow in this medium even under aerobic conditions. The small amount of agar used in the medium favors the growth of aerobes and anaerobes in the medium, even if sodium thioglycollate is deleted from the medium. Sodium thioglycollate act as a reducing agent and neutralizes the toxic effects of mercurial preservatives and peroxides formed in the medium, thereby promoting anaerobiosis, and making the medium suitable to test materials containing heavy metals. Any increase in the oxygen content is indicated by a colour change of redox indicator, resazurin to red. The small amount of agar helps in maintaining low redox potential for stabilizing the medium.