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MCDB 153 Medium w/ Trace elements, L-Glutamine and 28mM HEPES Buffer w/o NaHCO3

MCDB 153 Medium w/ Trace elements, L-Glutamine and 28mM HEPES Buffer w/o NaHCO3

₹ 15,920.00 *
  • Brand: HIMEDIA
  • Catalog No.: AT135-10X1L
  • Quantity/Unit: 10x1lt/EA
  • Usually Shipped in: 2-3 Weeks

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Min Orderable Qty : 1 Pack

For lab/research use only, unless otherwise specified


MCDB 153 Medium With Trace elements, L- Glutamine and 28mM HEPES buffer Without Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 5.1 -5.7 whereas the pH with Sodium Bicarbonate is between 6.4 -7.0. The osmolality without Sodium Bicarbonate is in the range of 300.00 -340.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 320.00 -360.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Ammonium metavanadate (0.000585)

Calcium chloride dihydrate (4.41)

Cupric sulphate pentahydrate (0.0027)

Disodium hydrogen phosphate anhydrous (284.09)

Ferrous sulphate heptahydrate (1.39)

Magnesium chloride hexahydrate (122.00)

Manganese sulphate (0.000151)

Molybdic acid ammonium tetrahydrate (0.00124)

Nickel chloride (0.00012)

Potassium chloride (111.83)

Sodium acetate anhydrous (301.53)

Sodium chloride (7599.00)

Sodium metasillicate nonahydrate (0.14)

Sodium selenite (0.0038)

Stannous chloride monohydrate (0.000113)

Zinc sulphate heptahydrate (0.14)

Amino acids

Choline chloride (13.96)

D-Biotin (0.015)

D-Ca-Pantothenate (0.24)

Folic acid (0.79)

Niacinamide (0.04)

Pyridoxine hydrochloride (0.06)

Riboflavin (0.038)

Thiamine hydrochloride (0.34)

Vitamin B12 (0.41)

myo-Inositol (18.02)

Adenine hydrochloride (30.88)

D-Glucose (1081.00)

HEPES buffer (6600.00)

Phenol red sodium salt (1.24)

Putrescine dihydrochloride (0.16)

Sodium pyruvate (55.00)

Thioctic acid (0.21)

Thymidine (0.73)

MCDB media were developed for the culture of specific cell types without a serum supplement. The media were supplemented with growth factors, hormones, trace elements, or low levels of dialyzed fetal bovine serum protein (FBSP). Each MCDB medium was formulated for a specific cell type. MCDB 105 and 110 were formulated for rapid clonal growth of normal human diploid cells. MCDB 131 medium was originally developed for the clonal growth of human micro-vascular endothelial cells (HMVEC). MCDB 151, 201 and 302 were originally developed for human keratinocytes, clonal growth of chick embryo fibroblasts and CHO cells. This product contains trace elements, L-glutamine and 28mM HEPES buffer. HEPES is a zwitterionic buffer having a pKa of 7.3 at 37C, which prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium.

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