MEM (Modified for Autoclaving) w/ Earles salts, NEAA w/o L-Glutamine, NaHCO3

MEM (Modified for Autoclaving) w/ Earles salts, NEAA w/o L-Glutamine, NaHCO3

₹ 490.00 *

Brand: HIMEDIA

Catalog No.: AT017A-1L

Quantity/Unit: 1lt/EA

Usually Shipped in: 2-3 Weeks

Qty:
Min Orderable Qty : 1 Pack

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Product Description

Minimum Essential Medium Eagle (MEM) (Modified for Autoclaving) With Earle's salts and NEAA Without L-Glutamine and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 4.1 -4.7 whereas the pH with Sodium Bicarbonate is between 7.3 -7.9. The osmolality without Sodium Bicarbonate is in the range of 240.00 -280.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 285.00 -325.00 Osm/Kg H20. It is advisable to store this product between 2-8⁰C. Media composition (in mg/L) is as follows:

• Calcium chloride dihydrate (265.00)

• Magnesium sulphate anhydrous (97.72)

• Potassium chloride (400.00)

• Sodium chloride (6800.00)

• Sodium dihydrogen phosphate anhydrous (122.00)

• Amino acids

• Choline bitartarate (1.80)

• D-Ca-Pantothenate (1.00)

• Folic acid (1.00)

• Nicotinamide (1.00)

• Pyridoxal hydrochloride (1.00)

• Riboflavin (0.10)

• Thiamine hydrochloride (1.00)

• i-Inositol (2.00)

• D-Glucose (1000.00)

• Phenol red sodium salt (6.30)

• Sodium succinate (100.00)

• Succinic acid (75.00)

Minimum Essential Medium (MEM) is a modification of Basal Medium Eagle (BME). Developed by Harry Eagle, it was designed to meet the specific nutritional requirements of certain subtypes of Hela cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely approximate the protein composition of cultured mammalian cells. MEM can be used either with Earle's salts or Hank's salts and can also be additionally supplemented with non-essential amino acids (NEAA). This medium can be further modified by eliminating calcium to facilitate growth of cells in suspension cultures. Autoclavable media offer a convenient alternative to membrane sterilized liquid medium. It is modified to include heat stable components to ensure that product efficacy is maintained after autoclaving. As L-glutamine is heat labile, it is not present in this formulation. 

 

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