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MEM w/ Earles salts, L-Glutamine, NEAA, Sodium pyruvate, HEPES Buffer w/o NaHCO3

MEM w/ Earles salts, L-Glutamine, NEAA, Sodium pyruvate, HEPES Buffer w/o NaHCO3

₹ 1,770.00 *
  • Brand: HIMEDIA
  • Catalog No.: AT182-5L
  • Quantity/Unit: 5lt/EA
  • Usually Shipped in: 2-3 Weeks

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Min Orderable Qty : 1 Pack

For lab/research use only, unless otherwise specified


Minimum Essential Medium Eagle (MEM) With Earle's salts, L-Glutamine, NEAA, Sodium pyruvate and 15mM HEPES buffer Without Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 4.9 -5.5 whereas the pH with Sodium Bicarbonate is between 6.7 -7.3. The osmolality without Sodium Bicarbonate is in the range of 255.00 -295.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 300.00 -340.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (265.00)

Magnesium sulphate anhydrous (97.72)

Potassium chloride (400.00)

Sodium chloride (6800.00)

Sodium dihydrogen phosphate anhydrous (122.00)

Amino acids

Choline chloride (1.00)

D-Ca-Pantothenate (1.00)

Folic acid (1.00)

Nicotinamide (1.00)

Pyridoxal hydrochloride (1.00)

Riboflavin (0.10)

Thiamine hydrochloride (1.00)

i-Inositol (2.00)

D-Glucose (1000.00)

HEPES buffer (3575.00)

Phenol red sodium salt (11.00)

Sodium pyruvate (110.00)

Minimum Essential Medium (MEM) is a modification of Basal Medium Eagle (BME). Developed by Harry Eagle, it was designed to meet the specific nutritional requirements of certain subtypes of Hela cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely approximate the protein composition of cultured mammalian cells. MEM can be used either with Earle's salts or Hank's salts and can also be additionally supplemented with non-essential amino acids (NEAA). This medium can be further modified by eliminating calcium to facilitate growth of cells in suspension cultures. HEPES is a zwitterionic buffer having a pKa of 7.3 at 37C, which prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium.

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