High grade Nitrate Broth is supplied by Micromaster. It is available in the form of a cream to yellow homogeneous free flowing powder which has a pH in the range of 6.6 to 7.0. For maximum efficiency, it must be stored below 30C in tightly closed container.

Quantity provided: 100g /pack

Nitrate Broth is recommended for detection of nitrate reduction by bacteria. This media is prepared in accordance with the formula published in Pure Culture Study of Bacteria of the Society of American Bacteriologist. Non-fermenters and other miscellaneous gram-negative bacilli vary in their ability to reduce nitrates. Some members of this group are capable of denitrification which is a reduction of nitrate to nitrogen gas. For the glucose fermenting gramnegative bacilli, the production of nitrogen gas from nitrate is an important differential test. Reduction of nitrate is generally an anaerobic respiration in which an organism derives its oxygen from nitrate. Members of Enterobacteriaceae characteristically reduce nitrate to nitrite which reacts with sulfanilic acid and N,N-dimethyl-1-naphthylamine to produce the red colour. This reaction is known as Griess reaction. If an organism grows rapidly and reduces nitrate actively, the test should be performed after an early incubation period since the nitrite may be further reduced to nitrogen. Potassium nitrate in the medium acts as a substrate for determining nitrate reduction by bacteria.