Pseudomonas Fluorescein Agar Plate is supplied by HiMedia. It contains 25ml of sterile ready prepared media in 90 mm disposable plates. This yellow coloured medium has a pH in the range of 6.8 to 7.2. Pseudomonas Fluorescein Agar is generally used for the detection of fluorescein production by Pseudomonas species. It is advisable to store this product between 15-25C. Composition (in gm/L) is as follows:

Casein enzymic hydrolysate (10.00)

Proteose peptone (10.00)

Dipotassium phosphate (1.50)

Magnesium sulphate (1.50)

Agar (15.00)

Pseudomonas Agar (For Fluorescein) is based on the formula by King et al and the modification in the U.S. Pharmacopeia. It is used for the detection of fluorescein (a water soluble, chloroform insoluble fluorescent pigment) produced by Pseudomonas species. The medium enhances the elaboration of fluorescein by Pseudomonas and inhibits the pyocyanin formation. The fluorescein pigment diffuses from the colonies of Pseudomonas into the agar and shows yellow fluorescent colouration. Some Pseudomonas strains produce small amount of pyocyanin which result in yellow-green colour. Casein enzymic hydrolysate and proteose peptone present in the media provide the essential nitrogenous nutrients, carbon, sulphur and trace elements for the growth of Pseudomonas. Dipotassium phosphate buffers the medium while magnesium sulphate supplies the necessary cations for the activation of fluorescein production. Salt concentration exceeding 2