High grade Pseudomonas Isolation Agar Base is supplied by Micromaster. It is available in the form of a white to cream homogeneous free flowing powder with a pH in the range of 6.8 to 7.2. For maximum efficiency, it must be stored below 30C in tightly closed container.

Quantity provided: 500g /pack

Pseudomonas Isolation Agar Base is used for selective isolation and identification of Pseudomonas aeruginosa from clinical and non-clinical specimens. Pseudomonas aeruginosa is an important human pathogen commonly found in nosocomial infections. It successfully combines adaptability to a variety of moist environments with a collection of potent virulence factors. Pseudomonas Isolation Agar Base, used for the selective isolation and identification of P. aeruginosa, is a modification of Medium A, originally formulated by King, Ward and Raney. The medium contains pigment-enhancing components and the selective agents, triclosan which selectively inhibits non-pseudomonads. The pigment-enhancers i.e. potassium sulphate and magnesium chloride enhance the blue or blue-green pigment production by P. aeruginosa, thus aiding in its identification. Peptic digest of animal tissue provides nitrogenous compounds and other essential growth nutrients. Glycerol is a source of energy and promotes pyocyanin i.e. pigment production which is characteristic of Pseudomonas. Potassium sulphate and magnesium chloride enhance pyocyanin production. Triclosan selectively inhibits gram-positive and gram-negative bacteria but Pseudomonas species are resistant to it. Presumptive Pseudomonas should be confirmed by performing biochemical tests, as some strains of Pseudomonas do not produce pyocyanin.