High grade TCBS Agar is supplied by Micromaster. It is available in the form of a light yellow to light tan homogenous free flowing powder with a pH in the range of 8.4 to 8.8. This product should be stored between 10-30C in a tightly closed container. Once opened, it should be properly stored, after tightly capping the bottle. Due to the hygroscopic nature of the product, there could be chances of lump formation which can be avoided by proper storage. Store in dry ventilated area protected from extremes of temperature and sources of ignition.

• Quantity provided: 100 g/pack

TCBS Agar is recommended for the selective isolation and cultivation of Vibrio cholerae and other enteropathogenic Vibrios causing food poisoning from clinical and food specimen. TCBS Agar was developed by Kobayashi et al, who modified the selective medium of Nakanishi. TCBS Agar is also recommended by APHA for the selective isolation of V. cholerae and V.parahaemolyticus. Enrichment in Alkaline Peptone Water, followed by isolation on TCBS Agar is routinely used for isolation of V.cholerae. Proteose peptone and yeast extract provide nitrogenous compounds, vitamin B complex and other essential growth nutrients. Bile, a derivative of bile salts and sodium citrate inhibit gram-positive bacteria and coliforms. Sodium thiosulphate serves as a good source of sulphur, which in combination with ferric citrate detects the production of hydrogen sulphide. For the metabolism of Vibrios, sucrose is added as a fermentable carbohydrate. Vibrio that is able to utilize sucrose will from yellow colonies. Bromothymol blue and thymol blue are the pH indicators. The alkaline pH of the medium improves the recovery of V.cholerae.