High grade Tergitol-7 Agar Base (Chapman) is supplied by Micromaster. It is available in the form of a cream to light green homogenous free flowing powder with a pH in the range of 6.7 to 7.1. It is advisable to store this product below 30C.

• Quantity provided: 100 g/pack

Tergitol-7 Agar Base is recommended for selective enumeration and identification coliform organisms. Tergitol-7 Agar was originally designed by Chapman and later modified by incorporating 2,3,5-Triphenyl Tetrazolium Chloride (TTC) into the medium. This medium is selective for the detection and enumeration of coliform organisms. Pollard has reported the selective bactericidal property of sodium heptadecyl sulphate (Tergitol-7). Kulp et al corroborated the use of Tergitol-7 Agar with TTC in routine analysis of water and Mossel used this medium for the examination of food materials. Proteose peptone and yeast extract serve as sources of carbon, nitrogen and other essential nutrients including vitamin B complex. Sodium heptadecyl sulphate (Tergitol-7) inhibits gram-positive bacteria and Proteus swarming and yields better recovery of coliforms. Bromo thymol blue is the pH indicator. Lactose fermenting organisms form yellow colonies with yellow zones while Klebsiella and Enterobacter form greenish yellow colonies. Lactose non-fermenters produce blue colonies. TTC is reduced by the bacterial cell except Escherichia coli and Enterobacter aerogenes to form formazan, a red coloured insoluble complex, thereby producing red coloured colonies.