Urea Indole Medium is supplied by the company, HIMEDIA.

This is a Urea Indole Medium which is a medium that actively contributes in differentiating micro-organisms especially Enterobactericeae on the basis of their ability to hydrolyze urea and indole production. On the basis of Urease and indole production and the transdeamination of tryptophan, identification of Enterobacteria is carried out using Urea Indole Medium. Proteus species from Salmonella and Shigella species is identified using this medium. L- Trypytophan is an essential amino acid present in the medium which is converted to skatole an d indole, detected by the addition of Kovacs Reagent (R008). Components like sodium chloride maintains the osmotic balance and phosphates helps in the buffering of the medium. Microorganisms that possess the enzyme urease hydrolyse urea, releasing ammonia, which is detected by the pH indicator phenol red . The alkalinility so developed imparts pink colour to the medium.

Product specifications:

• Catalog number: LQ126-25X3ML
• Brand: HIMEDIA
• Quantity: Pack of 25X3ML

Product composition:

• L- Tryptophan: 3.000gm/L
• Sodium chloride: 5.000gm/L
• Potassium phosphate, monobasic: 1.000gm/L
• Potassium phosphate, dibasic: 1.000gm/L
• Urea: 20.000gm/L
• Phenol red: 0.012 gm/L

Product features:

• Appearance: Sterile clear Urea Indole Medium in bottles
• Colour: Light orange coloured medium
• Quantity of medium: 3 ml of medium in bottles
• pH: 6.60- 7.00
• Sterility Testing: Passes release criteria
• Cultural Response: Cultural characteristics observed after incubation at 35-37C for 18-24 hours
• Storage and Shelf Life: Store between 2-8C. Use before expiry date on the label