X-GAL (For Molecular Biology),500 mg,98%
- Brand: RESEARCH-LAB FINE CHEM INDUSTRIES
- Catalog No.: 1576D 0500M
- CAS No: 7240-90-7
- Quantity/Unit: 500 mg/Bottle
- Usually Shipped in: 4-7 Days
Min Orderable Qty : 1 Pack
For lab/research use only, unless otherwise specified
X-gal by Research Lab Fine Chem Industries is a high grade reagent. Also known as 5-Bromo-4-chloro-3-indolyl--Dgalactopyranoside, this organic acid contains a galactose linked to a substituted indole. It yields an insoluble blue compound as a result of enzyme-catalysed hydrolysis. As an analogue of lactose, it is hydrolysed by beta-galactosidase to produce galactose and 5-bromo-4-chloro-3-hydroxyindole. The latter compound dimerizes and oxidizes to yield a blue coloured compound. The compound is stable under general conditions, however, it is incompatible with strong oxidizing agents. CAS No.: 7240-90-6 Quantity Provided: 500 mg per bottle Molecular formula: C14H15BrClNO6 Molecular weight: 408.63 Assay: 98 %X-gal has a variety of applications in chemical and biological sectors. This compound is extensively used to detect the presence active -galactosidase enzyme. This enables the lacZ gene to be used as a reporter gene in several applications. These majorly involve cloning procedures to differentiate successfully cloned products from the rest in a blue/white screening method. It can also be used in two-hybrid analysis to identify how proteins interact with each other. This reagent is suitable for industrial synthesis of products and research applications.