Antibiotic Assay Medium B is supplied by Micromaster. It is available in the form of a cream to yellow homogeneous free flowing powder which has a pH in the range of 7.1 to 7.5. It is advisable to store this product below 30⁰C in a tightly closed container.
• Quantity provided: 100 g/pack
Antibiotic Assay Medium B is used for the microbiological assay of Colistimethate sodium using Bordetella bronchiseptica ATCC 4617 and Escherichia coli ATCC 10536. Combination of casein enzymic hydrolysate and papaic digest of soyabean meal provides essential nutrients for the growth of test organisms. Dextrose provides fermentable source of carbon, and enhances the growth of test organims. Phosphates in the medium enhances buffering action and sodium chloride maintains osmotic equilibrium. It is recommended by various pharmacopoeia. Freshly prepared plates should be used for antibiotic assays. Test organisms are inoculated in sterile seed agar cooled to 40-45°C and spread evenly over the surface of solidified base agar. All conditions in the microbiological assay must be controlled carefully. The use of standard culture media in the test is one of the important steps for appropriate results.