Fluid Selenite Cystine Medium (Selenite Cystine Broth) is supplied by Micromaster. It is available as two parts where part A is composed of off-white to light yellow powder and part B consists of white to cream homogeneous free flowing powder. It has a pH in the range of 6.8 to 7.2. It is advisable to store this product below 30C.

Quantity provided: 100 g/pack

Fluid Selenite Cystine Broth is recommended as an enrichment medium for the isolation of Salmonellae from faeces, urine or other pathological materials. Selective inhibitory effects of selenite were first demonstrated by Klett. Guth used it to isolate Salmonella serotype Typhi. Leifson studied selenite and formulated a medium. Fluid Selenite Cystine Medium is a modification of Leifsons formula with added cystine. The formulation corresponds to that recommended by the AOAC for the detection of Salmonellae in foodstuff, particularly egg products. It is included by APHA, ISO and USP. Selenite Cystine Broth is useful for detecting Salmonella in the nonacute stages of illness when organisms occur in the faeces in low numbers and for epidemiological studies to enhance the detection of low numbers of organisms from asymptomatic or convalescent patients. Casein enzymic hydrolysate provide nitrogenous substances. Lactose maintains the pH in medium as selenite is reduced by bacterial growth and alkali is produced. An increase in pH lessens the toxicity of the selenite and results in overgrowth of other bacteria. The acid produced by bacteria due to lactose fermentation serves to maintain a neutral pH. Phosphate maintains a stable pH and also lessens the toxicity of selenite. L-cystine improves recovery of Salmonellae. Enriched broth is subcultured on solid medium.