Guanidine Hydrochloride - 1 KG

Application

Guanidine hydrochloride has been used:

• as a component of the extraction buffer for the extraction of proteoglycans
• in extraction during protein fractionation of ATDC5 cell lines
• as a chemical additive to study its effective absorbance spectra in structural analysis
• to incubate the device chip to release prostate-specific antigen and for regeneration of aptamer
• as a component of the 2,4-dinitrophenylhydrazine (DNPH) solution for detecting protein carbonyls by protein carbonylation study
• in RNA isolation to dissociate nucleoproteins and inhibit RNase

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.