HiPurA PCR Product and Gel Purification Combo Kit
₹ 34,960.00 *
Catalog No.: MB563-250PR
Usually Shipped in: 2-3 Weeks
Expiry Date : 01-Jan-1970
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HiPurA PCR Product and Gel Purification Combo Kit is supplied by HiMedia. The HiPurA PCR Product and Gel Purification Combo Kit simplifies the purification of nucleic acids from enzymatic reactions viz. PCR as well as from all grades of agarose gels in a single kit. The HiPurA DNA purification system combines the reversible nucleic acid binding properties of HiElute Miniprep Spin Column with an efficient buffer system, which eliminates contaminants such as proteins. This kit contains:
Combo Binding Buffer (CB)
Wash Solution Concentrate (WB)
Elution Buffer (ET) [10 mM Tris-Cl, pH 8.5]
HiElute Miniprep Spin Column (Capped) [in DBCA016 Collection Tube]
Collection Tube (Uncapped), Polypropylene(2.0ml)
Collection Tube, Polypropylene (2.0 ml)
The HiPurA PCR Product and Gel Purification Combo Kit is designed to purify DNA fragments from agarose gels. The simple procedure uses a silica-based spin column to purify DNA fragments (100bp-10kb). It also provides all components to perform a rapid and efficient removal of short primers, dNTPs, enzymes and salts from PCR fragments (100bp-10kb) as well. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound to the silica membrane, the contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt buffer or Molecular Biology Grade Water. Typically, recoveries are upto 80% for gel extraction and 80-95% for PCR product purification. Purified DNA using the HiPurA kit is free of proteins, dye and agarose, and is ready-to-use for a variety of applications including automated fluorescent DNA sequencing, PCR, in vitro transcription, restriction mapping, cloning and labeling.