HiPurA Quick Gel Purification Kit-MB539-20PR
₹ 3,655.00 *
Catalog No.: MB539-20PR
Usually Shipped in: 2-3 Weeks
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HiPurA Quick Gel Purification Kit is supplied by HiMedia. The HiPurA Quick Gel Purification Kit simplifies the extraction and purification of nucleic acids from a variety of sources. The HiPurA DNA purification system combines the reversible nucleic acid binding properties of HiElute Miniprep Spin Column (Capped) with an efficient buffer system, which eliminates contaminants such as proteins. The HiPurA Quick Gel Purification Kit simplifies recovery of DNA bands (100 bp-10 kb) from all grades of agarose gels. This kit contains:
Gel Wash Buffer Concentrate (BIW)
Gel Bind Buffer (HG)
Elution Buffer (ET) [10 mM Tris-Cl, pH 8.5]
HiElute Miniprep Spin Column (Capped) [in DBCA016 Collection Tube]
Collection Tube (Uncapped), Polypropylene (2.0 ml)
Collection Tube, Polypropylene (2.0 ml)
Gel purification of DNA is a common technique used for isolation of specific DNA fragments from reaction mixtures. However, most methods either fail to completely remove agarose leading to problems in downstream processing, or shearing of DNA resulting in very low yield. This kit uses the HiPurA technology to recover DNA bands of 100 bp-10 kb in length from all grades of agarose gel with yields exceeding 80%. The DNA band of interest is excised from the gel, dissolved in Gel Bind Buffer and applied to HiElute Miniprep Spin Column (Capped). The DNA is eluted with low salt buffer or Molecular Biology Grade Water after a rapid wash step. The product is suitable for downstream applications such as ligation, PCR, sequencing, restriction digestion and various labeling reactions.